IN VIVO ANTIDIABETIC ACTIVITY AND MECHANISM OF ACTION OF THREE CAMEROONIAN MEDICINAL PLANT EXTRACTS
DOI:
https://doi.org/10.29121/granthaalayah.v7.i8.2019.695Keywords:
Diabetes, C. Roseus, P. Americana, E. Globulus, Mechanism of ActionAbstract [English]
The aim of the present work is to study and compare antidiabetic activity of extracts from Catharentus roseus, Persea americana, and Eucalyptus globulus as well as their mechanism of action. The aqueous and methanolic plant extracts were respectively prepared by decoction and maceration. The phytochemical screening was done by standard methods. The extracts were subsequently tested in vivo on Wistar albino rats (Rattus norvegicus) with temporary hyperglycemia. The methanolic extract of P. americana leaves showing the best activity was selected for the antidiabetic test at doses 37.5, 75 and 150 mg/kg. For that purpose, permanent diabetes mellitus was induced in rats fasted overnight by intraperitoneal injection of alloxan (150 mg/kg). Hematological and biochemical parameters were evaluated from the cardiac puncture’s blood. The antidiabetic mechanism of action of the 6 extracts was performed by evaluating their inhibitory activity on alpha glucosidase, beta glucosidase and glucoamylase.
Phytochemical analysis of aqueous and methanolic extracts revealed that all extracts contain phenols, anthraquinones and tannins. On the other hand, no extract contains sterols. All extracts showed hypoglycemic activities. The antidiabetic tests showed that the methanolic extract of P. americana caused a decrease in blood sugar level in all the treated rats with the dose of 150 mg / kg of body weight being the most effective, without any adverse side effect in the animals. All the tested extracts showed an inhibitory activity against alpha glucosidase and glucoamylase enzymes and have no inhibitory activity on beta glucosidase.
The results of the present study demonstrate the antidiabetic properties of the tested plants through inhibitory effect on alpha glucosidase and glucoamylase enzymes with P. americana being the most active.
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Thaane T, Motala AA, McKune AJ. Effects of Short-Term Exercise in Overweight/Obese Adults with Insulin Resistance or Type 2 Diabetes: A Systematic Review of Randomized Controlled Trials. J Diabetes Metab. 2018; 9:12. DOI: https://doi.org/10.4172/2155-6156.1000816
International Diabetes federation (IDF). Diabetes Atlas, 6th edition, 2014, 160p.
Kirkman MS. Dual oral agent therapy for type 2 diabetes: Why don’t our patients stick with it? J Diabetes Complicat 2016; 30:1417-1418. DOI: https://doi.org/10.1016/j.jdiacomp.2016.08.014
Feudjio C, Njateng GSS, Kuiate J-K. Evaluation of Antidiabetic Activity of Aqueous Extract of Leaves from Phragmanthera capitata (Sprengel) S. Balle (Laurenthaceae) in Wistar Albino Rats. J Diseases Med Plants. 2018; 4:96-109. DOI: https://doi.org/10.11648/j.jdmp.20180404.11
Al-Shaqha WM, Khan M, Salam N, Azzi A, Chaudhary AA. Anti-diabetic potential of Catharanthus roseus Linn. and its effect on the glucose transport gene (GLUT-2 and GLUT-4) in streptozotocin induced diabetic wistar rats. BMC Complement Altern Med. 2015; 15:379 DOI: https://doi.org/10.1186/s12906-015-0899-6
Houacine C, Elkhawad AO, Ayoub SMH. A comparative study on the anti-diabetic activity of extracts of some Algerian and Sudanese plants. J Diabetes Endocrinol. 2012; 3:25-28.
Ezejiofor AN, Okorie A, Orisakwe OE. Hypoglycaemic and Tissue-Protective Effects of the Aqueous Extract of Persea Americana Seeds on Alloxan-Induced Albino Rats. Malays J Med Sci. 2013; 20:31-39.
Njateng GSS, Zaib S, Chimi YL, Feudjio C, Mouokeu RS, Gatsing D et al. Antidiabetic potential of methanol extracts from leaves of Piper umbellatum L. and Persea americana Mill. Asian Pacific J Trop Biomed. 2018; 8:160-165. DOI: https://doi.org/10.4103/2221-1691.227997
Bruneton J. Pharmacognosie, Phytochimie, Plantes médicinales. 3ième Edition. Paris: Lavoisier; 1999.
Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiol Res. 2001; 50:536-546
Njateng GSS, Gatsing D, Mouokeu RS, Kuiate J-R. Dermal Toxicity Study of an Antidermatophytic Oil-moistened Dichloromethane-Methanol (1:1 v/v) Stem Bark Extract of Polyscias fulva Hiern (Araliaceae) in Guinea Pigs Animal Model. Intern J Pharm. 2014; 4:53-60.
Ma H, Gaoh H, Sun L, Huang J., Xu X. Wu L. constituent with alpha glucosidase and advanced glycathion end- product formation inhibitory activities from salvia miltorrhiza bge. J nat Med. 2011; 65:37-42 DOI: https://doi.org/10.1007/s11418-010-0453-2
Doyle E, Egan M. Pharmacological Agents That Directly Modulate Insulin Secretion. Pharmacol reviews. 2003; 55:105-131. DOI: https://doi.org/10.1124/pr.55.1.7
Tian Y, Johnson G, Ashcroft S. Sulfonylureas enhance exocytosis from pancreatic beta-cells by a mechanism that does not involve direct activation of protein kinase C. Diabetes. 1998; 47:1722-1726. DOI: https://doi.org/10.2337/diabetes.47.11.1722
Essam A, Elberry A, Harraz F, Ghareib A, Nagy A, Gabr A. Antihyperglycemic and hypolipidaemic effects of the methanolic extract of Saudi mistletoe (Viscum schimperi Engl.), J Advanced Res. 2011;2:171-177. DOI: https://doi.org/10.1016/j.jare.2011.01.006
Guerci B, Bohme P, Kearney-Schwartz A, Zannad F, Drouin P. Endothelial dysfunction and type 2 diabetes. Diabetes Metab. 2001; 27:436-447.
Kim S, Hyun S, Choung S. Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice. J Ethnopharmacol. 2006; 104:119-123. DOI: https://doi.org/10.1016/j.jep.2005.08.059
Lukačínová A, Mojžiš R, Beňačka J, Keller T, Maguth P, Kurila L et al. Preventive Effects of Flavonoids on Alloxan-Induced Diabetes Mellitus in Rats. Acta Vet Brno. 2008; 77:175-82. DOI: https://doi.org/10.2754/avb200877020175
Silvestrini A, Pasqua G, Botta B, Monacelli B, Heijden R, Vonder Verpoorte R. Effects of alkaloid precursor feeding on a camptotheca acumisate cell line. Plant Physiol Biochem. 2002; 40:749-753. DOI: https://doi.org/10.1016/S0981-9428(02)01436-5
Li W, Zheng H, Bukuru J, DeKimpe N. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J Ethnopharmacol. 2004; 92:1-21. DOI: https://doi.org/10.1016/j.jep.2003.12.031
Wen X, Sun H, Liu J, Cheng K, Zhang P, Zhang L, et al. Naturally occurring pentacyclic triterpenes as inhibitors of glycogen phosphorylase: synthesis, structure− activity relationships, and X-ray crystallographic studies†. J med chem. 2008;51;3540-3554. DOI: https://doi.org/10.1021/jm8000949
Nakashima N, Kimura I, Kimura M, Matsura H. Isolation of pseudoprototimosaponin AIII from rhizomes of anemarrhenaasphodeloides and its hypoglycemic activity in steptozotocin-induced diabetic mice. J Nat Prod. 1993; 56:345-350 DOI: https://doi.org/10.1021/np50093a006
Tiwar A, Rao J. Diabetic mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects. Curr Sci. 2002; 83:30-37.
N'guessan K, Amoikon K, Soro D. Effect of Aqueous Extract of Persea Americana Seeds on the Glycemia of Diabetic Rabbits. Eur j Sci Res. 2009; 26:376-385.
Bouhouche I. Etude comparative de l’alloxane et de la streptozocine dans le diabète expérimental chez le rat blanc. Etude histologique du pancréas endocrine et la variation des paramètres sanguins. Mémoire de Magister en Biologie Animale, Université Constantine 1, Algérie, 2015;109 p.
Betterridge J. Lipid disorders in diabetes mellitus In Pickup J and Williams G. textbook of diabetes. London: Blackwell Science; 2002.
Eddouks M, Lemhadri A, Michel JB. Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats. J Ethnopharmacol. 2005; 98:345-350. DOI: https://doi.org/10.1016/j.jep.2005.01.053
Ravi Ram K, Ji S, Wolfner MF. Fates and targets of male accessory gland proteins in mated female Drosophila melanogaster. Insect Biochem Mol Biol. 2005; 35:1059-1071. DOI: https://doi.org/10.1016/j.ibmb.2005.05.001
Nkanu EE, Eno AE, Ofem OE, Imoru JO, Unoh FB. Effect of crude extract of Viscum album (mistletoe) on plasma lipids: an insight into its possible antihyperglycaemic and antihypertensive properties. Port Harcourt Med J. 2007; 1:171-177 DOI: https://doi.org/10.4314/phmedj.v1i3.38879
Sharma B, Viswanath G, Salunke R, Roy P. Effects of flavonoid-rich extract from seeds of Eugenia jambolana (L.) on carbohydrate and lipid metabolism in diabetic mice. Food Chem. 2008; 110:697-705. DOI: https://doi.org/10.1016/j.foodchem.2008.02.068
Bra B, Odetola A, Agomo P. Hypoglycemic and hypocholesterolemic potential of Persea americana leaf extracts, J Med Food. 2007; 10:356-360. DOI: https://doi.org/10.1089/jmf.2006.291
Du-Bois A. Action de l’intoxication alloxanique sur le foie de cobaye. Cellule and tissu research. 1954;40 :585-604
Gamberucci A, Konta L, Colucci A, Giunti R, Magyar J, Mandl J, et al. Green tea flavonols inhibit glucosidase II. Biochem Pharmacol. 2006; 72:640-646. DOI: https://doi.org/10.1016/j.bcp.2006.05.016
Lopiparo E, Scheib H, Frei N, Williamson G, Grigorov M, Chou C. Flavonoids for controlling starch digestion: structural requirements for inhibiting human alpha-amylase. J Med Chem. 2008; 51:3555-3561. DOI: https://doi.org/10.1021/jm800115x
Chattopadhyay RR. A comparative evaluation of some blood sugar lowering agents of plant origin. J Ethnopharmacol. 1999; 67:367-372. DOI: https://doi.org/10.1016/S0378-8741(99)00095-1
McDougall G, Shpiro F, Dobso P, Smith P, Blake A, Stewart D. Different polyphenolic components of soft fruits inhibit alpha-amylase and alpha-glucosidase. J Agric Food Chem. 2005; 3:2760-2766. DOI: https://doi.org/10.1021/jf0489926
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