IN VITRO DIRECT MULTIPLE SHOOT INDUCTION FROM LEAF EXPLANTS OF SOLANUM PUBESCENS WILLD

Ayyadurai V; Ramar K

doi:10.29121/granthaalayah.v4.i12SE.2016.2475

Authors

Ayyadurai.V PG and Research Department of Botany,National College (Autonomous), Tiruchirappalli-620 001, TamilNadu, India
Ramar.K PG and Research Department of Botany,National College (Autonomous), Tiruchirappalli-620 001, TamilNadu, India

DOI:

https://doi.org/10.29121/granthaalayah.v4.i12SE.2016.2475

Keywords:

Multiple Shoots, Solanum pubescens, MS Medium, BAP and GA3

Abstract [English]

Efficientin Vitro direct multiple shoot regeneration from Solanum pubescens was achieved from leaf explants on MS medium Sublimated with B5 vitamins and different concentrations and different combinations of PGRs like BAP, NAA and GA3. The maximum numbers of multiple shoots were achieved from leaf explants on 3.0 mg/l BAP + 1.0mg/l GA3. The regenerated shoots were transferred in to half strength MS medium fortified with IBA for root induction. Rooted plantlets were successfully acclimatized. This new and transfer into the field Conditions. Standardized and reproducible protocol useful the mass propagation of Solanum pubescens.

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References

Sumalatha P, Hemamalini K, Shwetha R, Uma Vasi Reddy. Antinociceptive screening of methanol extract of Solanum Pubescens. Int J Pharm, 4(2), 149-51, 2013. DOI: https://doi.org/10.7897/2230-8407.04825

Amin MM, Sawhney SS, Jassal SMM. Qualitative and quantitative analysis of phytochemicals of Taraxacumofficinale. Wudpecker, JPharmaPharmocol, 2(1), 001 – 005, 2013.

Rani G., Virk G.S. and Nagpal A. Callus induction and plantlet regeneration in Withaniasomnifera(L.) Dunal. In vitro cell Dev. Biol., Plant, 39(5), 468-474, 2003. DOI: https://doi.org/10.1079/IVP2003449

Sharma M.M. Ali D.J. and Batra A. Plant regeneration through in vitrosomatic embryogenesis in AshwagandhaWithaniasomnifera(L.) Dunal.Researcher, 2 (03), 1-6, 2010.

Rani G. and Grover I.S. In vitro callus induction and plant regeneration studies in Withaniasomnifera. Plant Cell Tissue and Organ Culture, 57, 23-27, 1999. DOI: https://doi.org/10.1023/A:1006329532561

Joshi A.G. and Padhya M.A. Shoot regeneration from leaf explants of Withaniasomnifera(L.) Dunal. Not. Sci.Biologicae, 2(01), 63-65, 2010. DOI: https://doi.org/10.15835/nsb213609

Sivanesan I. direct regeneration from apical bud explants of Withaniasomnifera(L.) Dunal. Ind. J. of Biotech, 6, 125-127, 2007.

Murashige T and Skoog F, A revised medium for rapid growth and bioassays with Tobacco tissue cultures, Physiol Plant, 15(3), 473-497, 1962. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Gamborg OL, Miller RA and Ojima O, Nutrient requirements of suspension cultures of soybean root cell, Exp. Cell Res, 50, 151-158, 1968. DOI: https://doi.org/10.1016/0014-4827(68)90403-5

Ramar K and v. Ayyadurai. In vitro direct multiple shoot induction and flower initiation of physalis minima. An important medicinal plant.Journal of international academic research for multidisciplinary, 1(1), 119-124, 2015.

Rathore, M.S.,Rathore, M.S., Shekhawat, N.S. Ex vivo implications of phytohormones on various in vitro responses in Leptadeniareticulata (Retz.) Wight. And arn an endangered plant. Environmental and Experimental Botany, 86, 86–93, 2013a. DOI: https://doi.org/10.1016/j.envexpbot.2010.05.009

Rathore, N.S., Rathore, N., Shekhawat, N.S., In vitro flowering and seed production in regenerated shoots of Cleome viscosa. Industrial Crops and Products, 50, 232–236, 2013b. DOI: https://doi.org/10.1016/j.indcrop.2013.07.032

Phulwaria, M., Shekhawat, N.S., Rathore, J.S., Singh, R.P., An efficient in vitro regeneration and ex vitro rooting of CeropegiabulbosaRoxb. A threatened and pharmaceutical important plant of Indian the Desert. Industrial Crops and Products,42, 25–29, 2013. DOI: https://doi.org/10.1016/j.indcrop.2012.05.013

Patel, A.K., Phulwaria, M., Rai, M.K., Gupta, A.K., Shekhawat, S., Shekhawat, N.S., In vitro propagation and ex vitro rooting of Carallumaedulis (Edgew.) Benth. &Hook. f. An endemic and endangered edible plant species of the Desert. ScientiaHorticulturae, 165, 175–180, 2014. DOI: https://doi.org/10.1016/j.scienta.2013.10.039

Ahmed, G., Roy, P.K., Mamum, A.N., High frequency shoots regeneration from nodal and shoots tip explants in Holarrhenaantidysenterica Wall. Indian Journal of Experimental Biology, 39, 1322–1324, 2001.

Ferdousi, B., Khazi, D.I.M., Paul, R.N., Mehendi, M., Shymole, R., In vitro propagation of emetic nut Randiadumetorum Lam. Indian Journal of Experimental Biology, 41, 1479–1489, 2003.

Kumar, R., Sharma, K., Agarwal, V., In vitro clonal propagation of Holarrhenaantidysenterica Wall. In Vitro Cellular and Developmental Biology Plant, 41 (2), 137–144, 2005. DOI: https://doi.org/10.1079/IVP2004624

Shirin, F., Rana, P., In vitro clonal propagation of mature Tectonagrandis L. through axillary bud proliferation. Journal of Forestry Research, 10, 465–469, 2005. DOI: https://doi.org/10.1007/s10310-005-0173-8

Shekhawat, M.S., Shekhawat, N.S., Micro propagation of Arnebiahispidissima(Lehm). DC. and production of alkannin from callus and cell suspension culture. ActaPhysiologiaePlantarum, 33, 1445–1450, 2011. DOI: https://doi.org/10.1007/s11738-010-0680-x

Shekhawat, M.S., Kannan, N., Manokari, M., Ramanujam, M.P., An efficient micro propagation protocol for high-frequency plantlet regeneration from liquid culture of nodal tissues in a medicinal plant, Turneraulmifolia L. Journal of Sustainable Forestry,33, 327–336, 2014. DOI: https://doi.org/10.1080/10549811.2013.847793

Sreeranjini, S., Siril, E.A., Field performance and genetic fidelity evaluation of micro propagated Morindacitrifolia L. Indian Journal of Biotechnology, 13, 121–130, 2014.